Nanoparticles strengthen intracellular tension and retard cellular migration.

Tay, Chor Yong; Cai, Pingqiang; Setyawati, Magdiel I; Fang, Wanru; Tan, Lay Poh; Hong, Catherine H L; Chen, Xiaodong; Leong, David Tai · Nano Lett · 2014

basic_science · Level V

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Abstract

Nanoparticles can have profound effects on cell biology. Here, we show that after TiO2, SiO2, and hydroxyapatite nanoparticles treatment, TR146 epithelial cell sheet displayed slower migration. Cells after exposure to the nanoparticles showed increased cell contractility with significantly impaired wound healing capability however without any apparent cytotoxicity. We showed the mechanism is through nanoparticle-mediated massive disruption of the intracellular microtubule assembly, thereby triggering a positive feedback that promoted stronger substrate adhesions thus leading to limited cell motility.

Medical subject headings