Drift time-specific collision energies enable deep-coverage data-independent acquisition proteomics.
basic_science · Level V
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- Record sourced from PubMed, PMID 24336358.
- Also identified by DOI 10.1038/nmeth.2767.
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Abstract
We present a data-independent acquisition mass spectrometry method, ultradefinition (UD) MS(E). This approach utilizes ion mobility drift time-specific collision-energy profiles to enhance precursor fragmentation efficiency over current MS(E) and high-definition (HD) MS(E) data-independent acquisition techniques. UDMS(E) provided high reproducibility and substantially improved proteome coverage of the HeLa cell proteome compared to previous implementations of MS(E), and it also outperformed a state-of-the-art data-dependent acquisition workflow. Additionally, we report a software tool, ISOQuant, for processing label-free quantitative UDMS(E) data.
Medical subject headings
- Ions
- Peptide Fragments
- Proteome
- Proteomics
- Tandem Mass Spectrometry