Whole blood cells loaded with messenger RNA as an anti-tumor vaccine.

Phua, Kyle K L; Boczkowski, David; Dannull, Jens; Pruitt, Scott; Leong, Kam W; Nair, Smita K · Adv Healthc Mater · 2014

basic_science · Level V

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Abstract

The use of a cell-based vaccine composed of autologous whole blood cells loaded with mRNA is described. Mice immunized with whole blood cells loaded with mRNA encoding antigen develop anti-tumor immunity comparable to DC-RNA immunization. This approach offers a simple and affordable alternative to RNA-based cellular therapy by circumventing complex, laborious and expensive ex vivo manipulations required for DC-based immunizations.

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