Quantitative single-cell RNA-seq with unique molecular identifiers.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24363023.
- Also identified by DOI 10.1038/nmeth.2772.
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Abstract
Single-cell RNA sequencing (RNA-seq) is a powerful tool to reveal cellular heterogeneity, discover new cell types and characterize tumor microevolution. However, losses in cDNA synthesis and bias in cDNA amplification lead to severe quantitative errors. We show that molecular labels--random sequences that label individual molecules--can nearly eliminate amplification noise, and that microfluidic sample preparation and optimized reagents produce a fivefold improvement in mRNA capture efficiency.
Medical subject headings
- High-Throughput Nucleotide Sequencing
- RNA, Messenger
- Sequence Analysis, RNA