Determination of HER2 amplification status on tumour DNA by digital PCR.
case_control · Level III
Where this comes from
- Record sourced from PubMed, PMID 24386193.
- Also identified by DOI 10.1371/journal.pone.0083409 and PMC identifier 3873285.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromosome 17 reference probe. We assessed a HER2:EFTDU2 ratio by digital PCR assay in the microdissected DNA from 18 HER2 amplified and 58 HER2 non-amplified cancers. The HER2:EFTUD2 ratio had high concordance with conventionally defined HER2 status with a sensitivity of 100% (18/18) and a specificity of 98% (57/58). The HER2:EFTUD2 digital PCR assay has potential to accurately assess HER2 amplification status.
Medical subject headings
- Gene Amplification
- Neoplasms
- Erb-b2 Receptor Tyrosine Kinases