Light-dependent and circadian transcription dynamics in vivo recorded with a destabilized luciferase reporter in Neurospora.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24391804.
- Also identified by DOI 10.1371/journal.pone.0083660 and PMC identifier PMC3423521.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We show that firefly luciferase is a stable protein when expressed at 25 °C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription dynamics associated with the Neurospora circadian clock and its blue-light photosensory system over the course of several days. We show that the tool is suitable to faithfully monitor rapid, but also subtle changes in transcription in a medium to high throughput format.