SNAP-tag technology optimized for use in Entamoeba histolytica.
basic_science · Level V
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- Record sourced from PubMed, PMID 24391864.
- Also identified by DOI 10.1371/journal.pone.0083997 and PMC identifier PMC2850560.
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Abstract
Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica. The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. We further demonstrate the capabilities of this system using super resolution microscopy, done for the first time in E. histolytica.