Single-molecule site-specific detection of protein phosphorylation with a nanopore.
basic_science · Level V
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- Record sourced from PubMed, PMID 24441471.
- Also identified by DOI 10.1038/nbt.2799 and PMC identifier 4391620.
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Abstract
We demonstrate single-molecule, site-specific detection of protein phosphorylation with protein nanopore technology. A model protein, thioredoxin, was phosphorylated at two adjacent sites. Analysis of the ionic current amplitude and noise, as the protein unfolds and moves through an α-hemolysin pore, enables the distinction between unphosphorylated, monophosphorylated and diphosphorylated variants. Our results provide a step toward nanopore proteomics.
Medical subject headings
- Biotechnology
- Nanopores
- Phosphorylation
- Proteins