Continuous throughput and long-term observation of single-molecule FRET without immobilization.
basic_science · Level V
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- Record sourced from PubMed, PMID 24441935.
- Also identified by DOI 10.1038/nmeth.2809.
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Abstract
We present an automated microfluidic platform that performs multisecond observation of single molecules with millisecond time resolution while bypassing the need for immobilization procedures. With this system, we confine biomolecules to a thin excitation field by reversibly collapsing microchannels to nanochannels. We demonstrate the power of our method by studying a variety of complex nucleic acid and protein systems, including DNA Holliday junctions, nucleosomes and human transglutaminase 2.
Medical subject headings
- Fluorescence Resonance Energy Transfer
- Microfluidics