Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24464288.
- Also identified by DOI 10.1038/nmeth.2816 and PMC identifier 3943662.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We combine super-resolution localization fluorescence microscopy with transmission electron microscopy of metal replicas to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. We validate robust correlation on the scale of 20 nm by imaging endogenous clathrin (in two and three dimensions) and apply the method to find the previously unknown three-dimensional position of the endocytic protein epsin on clathrin-coated structures at the plasma membrane.
Medical subject headings
- Gold
- Membrane Proteins
- Microscopy, Electron, Transmission
- Microscopy, Fluorescence
- Nanotubes