Toxicity assessment of silica coated iron oxide nanoparticles and biocompatibility improvement by surface engineering.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24465736.
- Also identified by DOI 10.1371/journal.pone.0085835 and PMC identifier 3897540.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We have studied in vitro toxicity of iron oxide nanoparticles (NPs) coated with a thin silica shell (Fe3O4/SiO2 NPs) on A549 and HeLa cells. We compared bare and surface passivated Fe3O4/SiO2 NPs to evaluate the effects of the coating on the particle stability and toxicity. NPs cytotoxicity was investigated by cell viability, membrane integrity, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) assays, and their genotoxicity by comet assay. Our results show that NPs surface passivation reduces the oxidative stress and alteration of iron homeostasis and, consequently, the overall toxicity, despite bare and passivated NPs show similar cell internalization efficiency. We found that the higher toxicity of bare NPs is due to their stronger in-situ degradation, with larger intracellular release of iron ions, as compared to surface passivated NPs. Our results indicate that surface engineering of Fe3O4/SiO2 NPs plays a key role in improving particles stability in biological environments reducing both cytotoxic and genotoxic effects.
Medical subject headings
- Coated Materials, Biocompatible
- Ferric Compounds
- Materials Testing
- Nanoparticles
- Nanotechnology
- Silicon Dioxide
- Toxicity Tests