New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24466046.
- Also identified by DOI 10.1371/journal.pone.0086349 and PMC identifier 3899247.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
MADS genes encode a family of transcription factors, some of which control the identities of floral organs in flowering plants. Most of the MADS-box genes in fern have been cloned and analyzed in model plants, such as Ceratopteris richardii and Ceratopteris pteridoides. In this study, a new MADS-box gene, DfMADS1(GU385475), was cloned from Dryopteris fragrans (L.) Schott to better understand the role of MADS genes in the evolution of floral organs. The full-length DfMADS1 cDNA was 973 bp in length with a 75 bp 5'-UTR and a 169 bp 3'-UTR. The DfMADS1 protein was predicted to contain a typical MIKC-type domain structure consisting of a MADS domain, a short I region, a K domain, and a C-terminal region. The DfMADS1 protein showed high homology with MADS box proteins from other ferns. Phylogenetic analysis revealed that DfMADS1 belongs to the CRM1-like subfamily. RT-PCR analysis indicated that DfMADS1 is expressed in both the gametophytes and the sporophytes of D. fragrans.
Medical subject headings
- Ferns
- MADS Domain Proteins