Single cell transfection with single molecule resolution using a synthetic nanopore.

Kurz, Volker; Tanaka, Tetsuya; Timp, Gregory · Nano Lett · 2014

basic_science · Level V

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Abstract

We report the development of a single cell gene delivery system based on electroporation using a synthetic nanopore, that is not only highly specific and very efficient but also transfects with single molecule resolution at low voltage (1 V) with minimal perturbation to the cell. Such a system can be used to control gene expression with unprecedented precision--no other method offers such capabilities.

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