A synthetic luciferin improves bioluminescence imaging in live mice.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24509630.
- Also identified by DOI 10.1038/nmeth.2839 and PMC identifier 4026177.
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Abstract
Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.
Medical subject headings
- Benzothiazoles
- Luminescent Measurements
- Neuroimaging