A mass spectrometry-based hybrid method for structural modeling of protein complexes.
basic_science · Level V
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- Record sourced from PubMed, PMID 24509631.
- Also identified by DOI 10.1038/nmeth.2841 and PMC identifier 3972104.
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Abstract
We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.
Medical subject headings
- Mass Spectrometry
- Models, Molecular
- Protein Conformation
- Proteins