2PE-STED microscopy with a single Ti:sapphire laser for reduced illumination.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24516661.
- Also identified by DOI 10.1371/journal.pone.0088464 and PMC identifier 3916425.
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Abstract
We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advantages of fluorescence yield increasing. The sub-diffraction-limit resolution was demonstrated using ATTO 425-tagged clathrin-coated vesicles.
Medical subject headings
- Aluminum Oxide
- Lasers
- Lighting
- Microscopy, Fluorescence
- Photons
- Titanium