Highly parallel transport recordings on a membrane-on-nanopore chip at single molecule resolution.
basic_science · Level V
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- Record sourced from PubMed, PMID 24524682.
- Also identified by DOI 10.1021/nl5002873 and PMC identifier 3953990.
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Abstract
Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250,000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread onto the nanopore-chip surface. Transport events of ligand-gated channels were recorded at single-molecule resolution by high-parallel fluorescence decoding.