Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24525480.
- Also identified by DOI 10.1038/ncomms4309 and PMC identifier 4061911.
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Abstract
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
Medical subject headings
- Crystallography
- Lipids
- Membrane Proteins