Magnetic ligation method for quantitative detection of microRNAs.

Liong, Monty; Im, Hyungsoon; Majmudar, Maulik D; Aguirre, Aaron D; Sebas, Matthew; Lee, Hakho; Weissleder, Ralph · Adv Healthc Mater · 2014

basic_science · Level V

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Abstract

A magnetic ligation method is utilized for the detection of microRNAs among a complex biological background without polymerase chain reaction or nucleotide modification. The sandwich probes assay can be adapted to analyze a panel of microRNAs associated with cardiovascular diseases in heart tissue samples.

Medical subject headings