Argonaute-bound small RNAs from promoter-proximal RNA polymerase II.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24581493.
- Also identified by DOI 10.1016/j.cell.2014.01.041 and PMC identifier 4111103.
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Abstract
Argonaute (Ago) proteins mediate posttranscriptional gene repression by binding guide miRNAs to regulate targeted RNAs. To confidently assess Ago-bound small RNAs, we adapted a mouse embryonic stem cell system to express a single epitope-tagged Ago protein family member in an inducible manner. Here, we report the small RNA profile of Ago-deficient cells and show that Ago-dependent stability is a common feature of mammalian miRNAs. Using this criteria and immunopurification, we identified an Ago-dependent class of noncanonical miRNAs derived from protein-coding gene promoters, which we name transcriptional start site miRNAs (TSS-miRNAs). A subset of promoter-proximal RNA polymerase II (RNAPII) complexes produces hairpin RNAs that are processed in a DiGeorge syndrome critical region gene 8 (Dgcr8)/Drosha-independent but Dicer-dependent manner. TSS-miRNA activity is detectable from endogenous levels and following overexpression of mRNA constructs. Finally, we present evidence of differential expression and conservation in humans, suggesting important roles in gene regulation.
Medical subject headings
- Gene Expression Regulation
- Promoter Regions, Genetic
- RNA Polymerase II
- RNA, Small Untranslated
- Transcription Elongation, Genetic