Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24584192.
- Also identified by DOI 10.1038/nmeth.2857.
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Abstract
Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations.
Medical subject headings
- CRISPR-Cas Systems
- Deoxyribonuclease I
- Endonucleases
- Genome, Bacterial