Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24584195.
- Also identified by DOI 10.1038/nmeth.2878 and PMC identifier 4040164.
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Abstract
Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.
Medical subject headings
- Spectrum Analysis, Raman