Heritable multiplex genetic engineering in rats using CRISPR/Cas9.
basic_science · Level V
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- Record sourced from PubMed, PMID 24598943.
- Also identified by DOI 10.1371/journal.pone.0089413 and PMC identifier 3943732.
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Abstract
The CRISPR/Cas9 system has been proven to be an efficient gene-editing tool for genome modification of cells and organisms. Multiplex genetic engineering in rat holds a bright future for the study of complex disease. Here, we show that this system enables the simultaneous disruption of four genes (ApoE, B2m, Prf1, and Prkdc) in rats in one-step, by co-injection of Cas9 mRNA and sgRNAs into fertilized eggs. We further observed the gene modifications are germline transmittable, and confirmed the off-target mutagenesis and mosaicism are rarely detected by comprehensive analysis. Thus, the CRISPR/Cas9 system makes it possible to efficiently and reliably generate gene knock-out rats.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Genetic Engineering