Single-molecule motions enable direct visualization of biomolecular interactions in solution.
basic_science · Level V
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- Record sourced from PubMed, PMID 24608179.
- Also identified by DOI 10.1038/nmeth.2882.
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Abstract
Biomolecular interactions are generally accompanied by modifications in size and charge of biomolecules at the nanometer scale. Here we describe a single-molecule method to sense these changes in real time based on statistical learning of diffusive and electric field-induced motion parameters of a trapped molecule in solution. We demonstrate the approach by resolving a monomer-trimer mixture along a protein dissociation pathway and visualizing the binding-unbinding kinetics of a single DNA molecule.
Medical subject headings
- DNA