Single molecule visualization and characterization of Sox2-Pax6 complex formation on a regulatory DNA element using a DNA origami frame.
basic_science · Level V
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- Record sourced from PubMed, PMID 24660747.
- Also identified by DOI 10.1021/nl4044949.
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Abstract
We report the use of atomic force microscopy (AFM) to study Sox2-Pax6 complex formation on the regulatory DNA element at a single molecule level. Using an origami DNA scaffold containing two DNA strands with different levels of tensile force, we confirmed that DNA bending is necessary for Sox2 binding. We also demonstrated that two transcription factors bind cooperatively by observing the increased occupancy of Sox2-Pax6 on the DNA element compared to that of Sox2 alone.
Medical subject headings
- DNA-Binding Proteins
- Eye Proteins
- Homeodomain Proteins
- Multiprotein Complexes
- Paired Box Transcription Factors
- Repressor Proteins
- SOXB1 Transcription Factors