Transcriptional profiling of cells sorted by RNA abundance.
basic_science · Level V
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- Record sourced from PubMed, PMID 24681693.
- Also identified by DOI 10.1038/nmeth.2910 and PMC identifier 4174458.
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Abstract
We have developed a quantitative technique for sorting cells on the basis of endogenous RNA abundance, with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high-fidelity transcriptome measurement of mouse induced pluripotent stem cells (iPSCs) isolated from a heterogeneous reprogramming culture. This method is broadly applicable to profiling transcriptionally distinct cellular states without requiring antibodies or transgenic fluorescent proteins.
Medical subject headings
- Cell Culture Techniques
- Gene Expression Profiling
- Induced Pluripotent Stem Cells
- RNA
- Transcription, Genetic