Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24706872.
- Also identified by DOI 10.1073/pnas.1314447111 and PMC identifier 3986197.
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Abstract
Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 µm, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient two-photon excitation. Two-photon MSIM gives resolution-doubled images with better sectioning and contrast in thick scattering samples such as Caenorhabditis elegans embryos, Drosophila melanogaster larval salivary glands, and mouse liver tissue.
Medical subject headings
- Lighting
- Microscopy
- Photons