Estimation of the relative sensitivity of qPCR analysis using pooled samples.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24722485.
- Also identified by DOI 10.1371/journal.pone.0093491 and PMC identifier 3983103.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The high sensitivity of qPCR makes it a desirable diagnostic method in epidemiological surveillance programs. However, due to high costs, the use of pooling has been suggested. In this paper, an algorithm based on the Montecarlo method has been designed and implemented. The algorithm had been tested in many different situations, and finally it was validated with a real dataset. Moreover, based on the results obtained and depending on pooling conditions, a drastic decrease of sensitivity is observed.
Medical subject headings
- Bacterial Infections
- Real-Time Polymerase Chain Reaction