Rapid adaptive optical recovery of optimal resolution over large volumes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24727653.
- Also identified by DOI 10.1038/nmeth.2925 and PMC identifier 4069208.
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Abstract
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
Medical subject headings
- Microscopy, Confocal
- Optics and Photonics