Scalable production of highly sensitive nanosensors based on graphene functionalized with a designed G protein-coupled receptor.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24742304.
- Also identified by DOI 10.1021/nl5006349 and PMC identifier 4025580.
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Abstract
We have developed a novel, all-electronic biosensor for opioids that consists of an engineered μ-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transistors with average mobility of ∼1500 cm(2) V(-1) s(-1) and yield exceeding 98%. The biosensor exhibits high sensitivity and selectivity for the target naltrexone, an opioid receptor antagonist, with a detection limit of 10 pg/mL.
Medical subject headings
- Biosensing Techniques
- Graphite
- Naltrexone
- Receptors, Opioid, mu