Mucosal adjuvant activity of IL-2 presenting spores of bacillus subtilis in a murine model of Helicobacter pylori vaccination.
basic_science · Level V
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- Record sourced from PubMed, PMID 24743850.
- Also identified by DOI 10.1371/journal.pone.0095187 and PMC identifier 3990602.
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Abstract
The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant B. subtilis spores presenting a fragment of the Helicobacter acinonychis UreB protein and expressing the ureB gene under vegetative promoter elicit a strong cellular immune response in orally immunized mice when co-administered with spores presenting IL-2. We show for the first time the successful application of two types of recombinant spores, one carrying an antigen and the other an adjuvant, in a single oral immunization.
Medical subject headings
- Adjuvants, Immunologic
- Bacillus subtilis
- Bacterial Vaccines
- Helicobacter Infections
- Helicobacter pylori
- Interleukin-2
- Vaccination