Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24747813.
- Also identified by DOI 10.1038/nmeth.2931 and PMC identifier 4141966.
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Abstract
Despite its many favorable properties as a sample support for biological electron microscopy, graphene is not widely used because its hydrophobicity precludes reliable protein deposition. We describe a method to modify graphene with a low-energy hydrogen plasma, which reduces hydrophobicity without degrading the graphene lattice. Use of plasma-treated graphene enables better control of protein distribution in ice for electron cryo-microscopy and improves image quality by reducing radiation-induced sample motion.
Medical subject headings
- Cryoelectron Microscopy
- Graphite
- Hydrogen
- Proteins