An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24747815.
- Also identified by DOI 10.1038/nmeth.2934.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We report a protein-fragment complementation assay (PCA) based on the engineered Deinococcus radiodurans infrared fluorescent protein IFP1.4. Unlike previous fluorescent protein PCAs, the IFP PCA is reversible, allowing analysis of spatiotemporal dynamics of hormone-induced signaling complexes in living yeast and mammalian cells at nanometer resolution. The inherently low background of infrared fluorescence permitted detection of subcellular reorganization of a signaling complex expressed at low abundance.
Medical subject headings
- Bacterial Proteins
- Fluorescent Dyes
- Infrared Rays
- Models, Molecular
- Spatio-Temporal Analysis