Microtiter plate-sized standalone chip holder for microenvironmental physiological control in gas-impermeable microfluidic devices.

Harink, Björn; Le Gac, Séverine; Barata, David; van Blitterswijk, Clemens; Habibovic, Pamela · Lab Chip · 2014

basic_science · Level V

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Abstract

We present a microtiter plate-sized standalone chip holder for precise control of physiological conditions inside closed microfluidic cell culture systems, made from gas-impermeable materials. Specifically, we demonstrate the suitability of the holder to support cell growth in a glass chip, to allow time-lapse imaging of live cells and the creation of a hypoxic environment, all relevant for applications in regenerative medicine research.

Medical subject headings