Targeted genomic rearrangements using CRISPR/Cas technology.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24759083.
- Also identified by DOI 10.1038/ncomms4728 and PMC identifier 4170920.
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Abstract
Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Gene Rearrangement