A red-shifted fluorescent substrate for aldehyde dehydrogenase.
basic_science · Level V
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- Record sourced from PubMed, PMID 24759454.
- Also identified by DOI 10.1038/ncomms4662 and PMC identifier 4063304.
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Abstract
Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH(pos) cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH(pos) murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.
Medical subject headings
- Aldehyde Dehydrogenase
- Coloring Agents
- Fluorescent Dyes
- Green Fluorescent Proteins