ThunderSTORM: a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging.

Ovesný, Martin; Křížek, Pavel; Borkovec, Josef; Svindrych, Zdeněk; Hagen, Guy M · Bioinformatics · 2014

basic_science · Level V

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Abstract

ThunderSTORM is an open-source, interactive and modular plug-in for ImageJ designed for automated processing, analysis and visualization of data acquired by single-molecule localization microscopy methods such as photo-activated localization microscopy and stochastic optical reconstruction microscopy. ThunderSTORM offers an extensive collection of processing and post-processing methods so that users can easily adapt the process of analysis to their data. ThunderSTORM also offers a set of tools for creation of simulated data and quantitative performance evaluation of localization algorithms using Monte Carlo simulations. ThunderSTORM and the online documentation are both freely accessible at https://code.google.com/p/thunder-storm/.

Medical subject headings