Photothermal effect for localized desorption of primary lymphocytes arrayed on an antibody/DNA-based biochip.

Leroy, Loïc; Bombera, Radoslaw; Engel, Elodie; Calemczuk, Roberto; Laplatine, Loïc; Baganizi, Dieu-donné R; Marche, Patrice N; Roupioz, Yoann et al. · Lab Chip · 2014

basic_science · Level V

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Abstract

This work proposes a miniaturized system able to perform multiple cell capture followed by cell-type selective release from a biochip surface. Unlabelled lymphocytes were first specifically captured onto a DNA array by antibody-DNA conjugates. The immobilized cells were subsequently released under spatiotemporal control within local heating generated by intense Surface Plasmon Resonance (SPR) produced by laser illumination.

Medical subject headings