Reversible protein inactivation by optogenetic trapping in cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24793453.
- Also identified by DOI 10.1038/nmeth.2940.
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Abstract
We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP.
Medical subject headings
- Light
- Optogenetics
- Proteins