CRISPR transcriptional repression devices and layered circuits in mammalian cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24797424.
- Also identified by DOI 10.1038/nmeth.2969 and PMC identifier 4228775.
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Abstract
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Genetic Engineering