The screening research of anti-inflammatory bioactive markers from different flowering phases of Flos Lonicerae Japonicae.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24809338.
- Also identified by DOI 10.1371/journal.pone.0096214 and PMC identifier 4014484.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Flos Lonicerae Japonicae (FLJ) is an important cash crop in eastern Asia, and it is an anti-inflammatory Traditional Chinese Medicine. There are large variations in the quality of the marketed FLJ products. To find marker ingredients useful for quality control, a tandem technology integrating ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF), principal component analysis (PCA), heat map analysis and hierarchical cluster analysis coupled with a NF-κB luciferase reporter gene assay were used to identify the different ingredients from the green bud, white bud, flowering stage and leaf stages, as well as to screen the anti-inflammatory activity of FLJ compositions. As flowering progressed, the anti-inflammatory effects of FLJ gradually decreased; however, chlorogenic acid, swertiamarin and sweroside should be used to evaluate the quality of FLJ products.
Medical subject headings
- Anti-Inflammatory Agents
- Lonicera
- Medicine, Chinese Traditional
- Plant Extracts