Study on the interaction of β-cyclodextrin and berberine hydrochloride and its analytical application.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24810601.
- Also identified by DOI 10.1371/journal.pone.0095498 and PMC identifier 4014474.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The fluorescence enhancement of berberine hydrochloride (BBH) as a result of complex with β-cyclodextrin (β-CD) is investigated. The mechanism of the inclusion was studied and discussed by spectrofluoremetry and infrared spectrograms. The results showed that a 1∶1 (β-CD: BBH) complex was formed with an apparent association constant of 4.23×10(2) L/mol. Based on the enhancement of the fluorescent intensity of berberine hydrochloride, a new spectrofluorimetric method for the determination of BBH in the presence of β-CD was developed. The linear range was 1.00∼4.00 µg/mL with the detection limit of 5.54 ng/mL. The proposed method was successfully applied to the determination of BBH in tablets.
Medical subject headings
- Berberine
- beta-Cyclodextrins