Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24813624.
- Also identified by DOI 10.1038/nmeth.2962.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.
Medical subject headings
- Crystallography
- Electron Transport Complex IV
- Lasers