Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography.
basic_science · Level V
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- Record sourced from PubMed, PMID 24813625.
- Also identified by DOI 10.1038/nmeth.2961 and PMC identifier 4081473.
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Abstract
Cryo-electron tomography (CET) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated cryo-PALM (photoactivated localization microscopy)-CET method for localizing objects within cryo-tomograms to beyond the diffraction limit of the light microscope. Using cryo-PALM-CET, we identified multiple and new conformations of the dynamic type VI secretion system in the crowded interior of Myxococcus xanthus.
Medical subject headings
- Bacterial Secretion Systems
- Cryoelectron Microscopy
- Electron Microscope Tomography
- Myxococcus xanthus