Selective detection and quantification of modified DNA with solid-state nanopores.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24821614.
- Also identified by DOI 10.1021/nl501340d.
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Abstract
We demonstrate a solid-state nanopore assay for the unambiguous discrimination and quantification of modified DNA. Individual streptavidin proteins are employed as high-affinity tags for DNA containing a single biotin moiety. We establish that the rate of translocation events corresponds directly to relative concentration of protein-DNA complexes and use the selectivity of our approach to quantify modified oligonucleotides from among a background of unmodified DNA in solution.
Medical subject headings
- DNA
- Nanopores