Myotube formation on gelatin nanofibers - multi-walled carbon nanotubes hybrid scaffolds.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24831971.
- Also identified by DOI 10.1016/j.biomaterials.2014.04.021.
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Abstract
Engineering functional muscle tissue requires the formation of densely packed, aligned, and mature myotubes. To enhance the formation of aligned myotubes with improved contractibility, we fabricated aligned electrospun gelatin multi-walled carbon nanotubes (MWNTs) hybrid fibers that were used as scaffolds for the growth of myoblasts (C2C12). The MWNTs significantly enhanced myotube formation by improving the mechanical properties of the resulting fibers and upregulated the activation of mechanotransduction related genes. In addition, the fibers enhanced the maturation of the myotubes and the amplitude of the myotube contractions under electrical stimulation (ES). Such hybrid material scaffolds may be useful to direct skeletal muscle cellular organization, improve cellular functionality and tissue formation.
Medical subject headings
- Gelatin
- Muscle Fibers, Skeletal
- Nanofibers
- Nanotubes, Carbon
- Tissue Scaffolds