Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24836920.
- Also identified by DOI 10.1038/nmeth.2964 and PMC identifier 4100252.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
Medical subject headings
- Calcium
- Imaging, Three-Dimensional
- Microscopy
- Neurons