Methods for multiplex template sampling in digital PCR assays.
basic_science · Level V
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- Record sourced from PubMed, PMID 24854517.
- Also identified by DOI 10.1371/journal.pone.0098341 and PMC identifier 4031183.
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Abstract
The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Three alternative assay approaches are presented for implementing MTS analysis of chromosome 21, providing a 10-fold concentration enhancement while preserving assay precision.
Medical subject headings
- Multiplex Polymerase Chain Reaction