Fluorogenic probes for live-cell imaging of the cytoskeleton.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24859753.
- Also identified by DOI 10.1038/nmeth.2972.
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Abstract
We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.
Medical subject headings
- Actins
- Cytoskeleton
- Fluorescent Dyes
- Microscopy, Confocal
- Tubulin