Gene delivery from supercharged coiled-coil protein and cationic lipid hybrid complex.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24875765.
- Also identified by DOI 10.1016/j.biomaterials.2014.05.005 and PMC identifier 4099184.
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Abstract
A lipoproteoplex comprised of an engineered supercharged coiled-coil protein (CSP) bearing multiple arginines and the cationic lipid formulation FuGENE HD (FG) was developed for effective condensation and delivery of nucleic acids. The CSP was able to maintain helical structure and self-assembly properties while exhibiting binding to plasmid DNA. The ternary CSP·DNA(8:1)·FG lipoproteoplex complex demonstrated enhanced transfection of β-galactosidase DNA into MC3T3-E1 mouse preosteoblasts. The lipoproteoplexes showed significant increases in transfection efficiency when compared to conventional FG and an mTat·FG lipopolyplex with a 6- and 2.5-fold increase in transfection, respectively. The CSP·DNA(8:1)·FG lipoproteoplex assembled into spherical particles with a net positive surface charge, enabling efficient gene delivery. These results support the application of lipoproteoplexes with protein engineered CSP for non-viral gene delivery.
Medical subject headings
- Gene Transfer Techniques
- Genetic Therapy
- Nerve Tissue Proteins